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Change Data Capture & Change Tracking Deep Dive - ppt download
Change Data Capture Change Tracking Deep Dive - ppt download
GE’s Electronic Metering Family - ppt video online download
GE s Electronic Metering Family - ppt video online download
Genome-wide analysis of RNA-guided dCas9 binding in Neuro-2a cells. (A)... | Download Scientific ...
Genome-wide analysis of RNA-guided dCas9 binding in Neuro-2a cells A Download Scientific
Addgene: CRISPR Guide
Addgene CRISPR Guide
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dcas9 capture enables analysis : dCas9 capture enables analysis of locus-specific chromatin-regulating proteome • dCas9 capture identifies high-resolution locus-specific long-range DNA interactions • Capture of disease-associated CREs uncovers mechanisms for β-globin disorders • Capture of super-enhancers reveals composition-based hierarchical structureCAPTURE: In Situ Analysis of Chromatin Composition of Endogenous Genomic Loci by Biotinylated dCas9. ... d dCas9 capture enables analysis of locus-specific chromat in-regulating proteome.dCas9 capture enables analysis of locus-specific chromatin-regulating proteome ... By combining dCas9 capture with chromatin interaction assays, we revealed locus-specific DNA interactions critical for regulatory function. In situ capture of SE constituents and disease-associated ...Resource In Situ Capture of Chromatin Interactions by Biotinylated dCas9 Xin Liu, 1,7Yuannyu Zhang, Yong Chen,2,7 Mushan Li,3,7 Feng Zhou,4,7,* Kailong Li, 1Hui Cao, Min Ni, 1Yuxuan Liu, Zhimin Gu, 1Kathryn E. Dickerson, Shiqi Xie,5 Gary C. Hon,5 Zhenyu Xuan,2 Michael Q. Zhang,2,6 Zhen Shao,3 and Jian Xu1,8,* 1Children’s Medical Center Research Institute, Department of Pediatrics, University ...Hybrid capture and Amplicon-Seq. The reset DKO dCas9–cat3a line was transduced with a pool a 10 sgRNAs (see also section on sgRNA cloning, sequences and coordinates). Upon Puromycin selection (2 ...CAPTURE: In Situ Analysis of Chromatin Composition of Endogenous Genomic Loci by Biotinylated dCas9 Xin Liu. Children's Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas.Unbiased analysis of the compositional structure of developmentally regulated or disease-associated CREs identifies new features of transcriptional regulation. Hence, CAPTURE provides a versatile platform to study genomic locus-regulating chromatin composition in a mammalian genome.Here, we employed ChIP-seq analysis followed by a targeted sequence capture approach to determine RNA-guided dCas9 specificity and off-target endonuclease activity on a genome-wide scale. We used two replicates for two different gRNA targets, which allowed us to identify gRNA-specific binding as well as binding events that occurdCas9-mCherry-APEX2 targets telomeres and enables restricted biotinylation of endogenous proteins. Fluorescence imaging of dSpyCas9-mCherry-APEX2 labeling in cells. Stable sgTelo and sgNS cells were labeled live or were only supplemented with H 2 O 2 as a no-labeling control. Cells were then fixed and stained with neutravidin conjugated with ...Genome-wide analysis of RNA-guided dCas9 binding in Neuro-2a cells. (A) Diagram of the Flag-tagged Cas9 double mutant (dCas9) used in ChIP-seq assay. dCas9 without an effector domain (dCas9-Flag) or fused to a KRAB repressor domain (dCas9-KRAB) are shown at top.Location of nuclear localization domain (NLS) is indicated in yellow, 3X Falg-tag is shown in orange and KRAB domain is high lighted ...
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